Emergency Pets Protocol – U.S. Clients

If you are outside of the U.S. click here for our International Emergency Instructions.

We recognize that the loss of a pet (or the consideration of euthanization) is an emotional time and many thoughts are on your mind.

If you do wish to have a Genetic Preservation (GP) of your pet, it is important to collect the necessary tissue samples promptly.   Below you will find instructions for collecting those samples.

While Genetic Preservation (GP) does not require you to clone your pet, if you would like to clone your pet Genetic Preservation (GP) is necessary.

If you have questions, please do not hesitate to call us at 1-888-876-6104.

 

General Information

Time is of the essence in a post-mortem situation. If the animal/tissue has been kept under optimal conditions post mortem — cool (approximately 4ºC), not frozen — then the maximum length of time we can accept the biopsy samples is 5 days post mortem. Storage at higher temperatures will decrease the chance of successful preservation and should be avoided. Keep in mind that each day the animal has been deceased minimizes the chance of a successful preservation. Please be aware the best way to assure a successful preservation is to have your animal biopsied while it is alive. These instructions are to be used for obtaining tissue for genetic preservation of cells from deceased animals. Tissue samples received will be used to establish cell cultures, and the resulting cells will be harvested and stored frozen.

SHIPPING

DO NOT ship samples on Friday as our lab does not receive shipments on the weekends. If samples are from a deceased animal and need to be received in lab as soon as possible, contact ViaGen for alternate instructions.  Do not ship samples on or close to holidays.

Sudden Death or Euthanasia

If the animal dies suddenly or if you must euthanize the animal, refrigerate but DO NOT FREEZE the animal until you perform the biopsy procedure. If euthanizing the animal, collect biopsy samples prior to euthanization. Complete the biopsy procedure and store each biopsy sample in a separate sterile tube containing sterile saline. Blood tubes without anticoagulant are appropriate. Store tubes in the refrigerator until ready to package and send out.  DO NOT FREEZE.

Items Required

Razor

Sterilizing Agent

Sterile Gauze

Scalpel Blade

Tweezers

Marker

Sterile Blood Tubes (without anticoagulant)

Sterile Saline

Ice Packs

Insulated Foam Cooler for Shipment

Newspaper or Bubble Wrap

Storage Agreement (if not previously signed)

Canine Statement of Health or Feline Statement of Health

 

Sample Types

Collect skin tissue samples from at least two different sites. Abdomen, inner leg and ear are acceptable biopsy sites.

We prefer to receive four independent samples from more than one biopsy site.

 

Aseptic Preparation of Biopsy Site

The biopsy area must be shaved and cleansed with a series of chlorhexidine and alcohol scrubs.

 

Tools and Technique

A sterile scalpel and forceps are required. Cut through all the layers including the epidermis, dermis, and the most superficial parts of the subcutis. Remove a total of 4 (2.0 cm X 2.0 cm) tissue samples. Using sterile forceps, place 4 tissue samples in tubes containing sterile saline (one sample per tube). Refrigerate samples until just  prior to shipping.

 

Labeling Instructions

Using a marker, clearly label one tube for each sample with the following:

1. Tissue Type

2. Collection Date

3. Date of Death

4. Species

5. Pet’s Name

6. Sex of the Pet

Shipment Procedure for Tissue Samples

1.  Place the ice packs in the bottom of an insulated foam cooler.

2. Wrap the sample in newspaper or bubble wrap, so they will not come in direct contact with the ice.

3. Place the samples on top of the ice packs.

4. Place any contracts in a bag and include in the box.

5. Seal the box and immediately send samples to ViaGen via FedEX® PRIORITY OVERNIGHT.